Human Soluble Cluster of differentiation 38 ELISA Kit | sCD38 elisa kit

Human Soluble Cluster of differentiation 38 (sCD38) ELISA Kit

Cat. Num. AAA284155

• Gene Names: CD38; ADPRC1; ADPRC 1
• Reactivity: Human
• Synonyms: Soluble Cluster of differentiation 38; Human Soluble Cluster of differentiation 38 (sCD38) ELISA Kit; sCD38 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CD38. No significant cross-reactivity or interference between Human CD38 and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for sCD38 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CD38 in samples. An antibody specific for CD38 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD38 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD38 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD38 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 38454326
• NCBI GeneID: 952
• NCBI Accession #: NP_001766.2
• NCBI GenBank Nucleotide #: NM_001775.3
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Cell surface; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.99.20; EC 3.2.2.6; Hydrolase; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: cell surface; integral component of membrane; membrane; nucleus; plasma membrane

Molecular Function: NAD(P)+ nucleosidase activity; NAD+ nucleosidase activity; phosphorus-oxygen lyase activity; transferase activity

Biological Process: B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; female pregnancy; NAD metabolic process; negative regulation of apoptosis; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of cell growth; positive regulation of insulin secretion; positive regulation of transcription, DNA-templated; positive regulation of vasoconstriction; response to drug; response to estradiol; response to hydroperoxide; response to hypoxia; response to progesterone; response to retinoic acid; signal transduction