Mouse Cathepsin L (CTSL) ELISA Kit | CTSL elisa kit

Mouse Cathepsin L (CTSL) ELISA Kit

Cat. Num. AAA450272

• Gene Names: Ctsl; fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• Reactivity: Mouse
• Synonyms: Cathepsin L (CTSL); Mouse Cathepsin L (CTSL) ELISA Kit; CTSL1; CATL; CTS-L; MEP; Cathepsin L1; Major excreted protein; CTSL elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTSL. No significant cross-reactivity or interference between CTSL and analogues was observed.
• Sequence Length: 334

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 23.7pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTSL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTSL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTSL in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CTSL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CTSL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTSL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CTSL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753558
• NCBI GeneID: 13039
• NCBI Accession #: NP_034114.1
• NCBI GenBank Nucleotide #: NM_009984.4
• UniProt Accession #: Q543M3; P06797
• Molecular Weight: 37,547 Da
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L
• NCBI Official Symbol: Ctsl
• NCBI Official Synonym Symbols: fs; MEP; nkt; CatL; Ctsl1; 1190035F06Rik
• NCBI Protein Information: cathepsin L1
• UniProt Protein Name: Cathepsin L
• UniProt Gene Name: Ctsl

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the activation peptide and the cathepsin L1 heavy and light chains. The mature enzyme appears to be important in embryonic development through its processing of histone H3 and may play a role in disease progression in a model of kidney disease. Homozygous knockout mice for this gene exhibit hair loss, skin thickening, bone and heart defects, and enhanced susceptibility to bacterial infection. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]