SCARB3 recombinant protein

Recombinant Human SCARB3 Protein (His tag)

Cat. Num. AAA2546068

• Gene Names: CD38; ADPRC1; ADPRC 1
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: SCARB3; Recombinant Human SCARB3 Protein (His tag); T10; CD38; SCARB3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 300

• Application Notes: The secreted recombinant human CD38 comprises 269 amino acids with a predicted molecular mass of 31.3 kDa. As a result of glycosylation, rhCD38 migrates as an approximately 43-45 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Val 43
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SCARB3 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

Description: A DNA sequence encoding the extracellular domain of human CD38 (NP_001766.2) (Val 43-Ile 300) with a C-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 38454326
• NCBI GeneID: 952
• NCBI Accession #: NP_001766.2
• NCBI GenBank Nucleotide #: NM_001775.3
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Cell surface; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.99.20; EC 3.2.2.6; Hydrolase; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: membrane

Molecular Function: NAD+ nucleosidase activity; phosphorus-oxygen lyase activity

Biological Process: B cell receptor signaling pathway; negative regulation of apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of transcription, DNA-dependent; response to drug