Human Protein S100-A7 ELISA Kit | S100A7 elisa kit

Human Protein S100-A7 ELISA Kit

Cat. Num. AAA764372

• Gene Names: S100A7; PSOR1; S100A7c
• Reactivity: Human
• Synonyms: Protein S100-A7; Human Protein S100-A7 ELISA Kit; S100A7/S100 Calcium Binding Protein A7/Protein S100-A7/Psoriasin/PSOR1/S100A7C/HID 5/HID5/HID-5/psoriasin 1; S100A7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100A7. No significant cross-reactivity or interference between S100A7 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.234-15ng/ml
• Sensitivity: <0.141ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A7 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A7 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-S100A7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-S100A7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the S100A7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of S100A7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 115298657
• NCBI GeneID: 6278
• NCBI Accession #: NP_002954.2
• NCBI GenBank Nucleotide #: NM_002963.3
• Molecular Weight: 11,471 Da
• NCBI Official Full Name: protein S100-A7
• NCBI Official Synonym Full Names: S100 calcium binding protein A7
• NCBI Official Symbol: S100A7
• NCBI Official Synonym Symbols: PSOR1; S100A7c
• NCBI Protein Information: protein S100-A7
• UniProt Protein Name: Protein S100-A7
• Protein Family: Protein
• UniProt Gene Name: S100A7
• UniProt Synonym Gene Names: PSOR1; S100A7C
• UniProt Entry Name: S10A7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A7: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; extracellular region; focal adhesion; nucleus

Molecular Function: calcium ion binding; protein binding; RAGE receptor binding; zinc ion binding

Biological Process: angiogenesis; defense response to Gram-negative bacterium; epidermis development; innate immune response; keratinocyte differentiation; response to lipopolysaccharide; response to reactive oxygen species; sequestering of metal ion