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Western Blot (WB) (FAM109A Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the FAM109A antibody detected the FAM109A protein (arrow).)

Rabbit anti-Human FAM109A Polyclonal Antibody | anti-FAM109A antibody

FAM109A Antibody (N-term)

Gene Names
FAM109A; SES1; IPIP27A
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FAM109A; Polyclonal Antibody; FAM109A Antibody (N-term); Sesquipedalian-1; Ses1; 27 kDa inositol polyphosphate phosphatase-interacting protein A; IPIP27A; anti-FAM109A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FAM109A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-31 amino acids from the N-terminal region of human FAM109A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
3-31
Sequence Length
262
Applicable Applications for anti-FAM109A antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
IHC-P~~1:10~50
Function
Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane.
Cellular Location
Early endosome. Recycling endosome. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle, clathrin- coated vesicle. Note=Interaction with OCRL may be crucial for targeting to endosome and to the trans-Golgi network. Also found on macropinosomes. Not detected in late endosomes, nor in lysosomes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(FAM109A Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the FAM109A antibody detected the FAM109A protein (arrow).)

Western Blot (WB) (FAM109A Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the FAM109A antibody detected the FAM109A protein (arrow).)

Immunohistochemistry (IHC)

(FAM109A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAM109A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (FAM109A Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAM109A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-FAM109A antibody
This gene encodes a protein that localizes to the endosome and interacts with the enzyme, inositol polyphosphate 5-phosphatase OCRL-1. Alternate splicing results in multiple transcript variants.
References
Swan, L.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(8):3511-3516(2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27215
NCBI Official Full Name
sesquipedalian-1 isoform 1
NCBI Official Synonym Full Names
family with sequence similarity 109, member A
NCBI Official Symbol
FAM109A
NCBI Official Synonym Symbols
SES1; IPIP27A
NCBI Protein Information
sesquipedalian-1
UniProt Protein Name
Sesquipedalian-1
Protein Family
UniProt Gene Name
FAM109A
UniProt Synonym Gene Names
Ses1; IPIP27A
UniProt Entry Name
SESQ1_HUMAN

NCBI Description

This gene encodes a protein that localizes to the endosome and interacts with the enzyme, inositol polyphosphate 5-phosphatase OCRL-1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

FAM109A: Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane. Belongs to the sesquipedalian family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 12q24.12

Cellular Component: recycling endosome; clathrin-coated vesicle; early endosome; trans-Golgi network

Molecular Function: protein binding; protein homodimerization activity

Biological Process: receptor recycling; endosome organization and biogenesis; retrograde transport, endosome to Golgi

Research Articles on FAM109A

Similar Products

Product Notes

The FAM109A fam109a (Catalog #AAA9207877) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 3-31. The FAM109A Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM109A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the FAM109A fam109a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM109A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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