Rabbit anti-Human NDUFS6 Polyclonal Antibody | anti-NDUFS6 antibody

NDUFS6, ID (NDUFS6, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, Complex I-13kD-A, NADH-ubiquinone oxidoreductase 13kD-A subunit) (Azide free) (HRP)

Cat. Num. AAA6324445

• Gene Names: NDUFS6; CI-13kA; CI13KDA; CI-13kD-A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFS6; Polyclonal Antibody; ID (NDUFS6; NADH dehydrogenase [ubiquinone] iron-sulfur protein 6; mitochondrial; Complex I-13kD-A; NADH-ubiquinone oxidoreductase 13kD-A subunit) (Azide free) (HRP); NADH-ubiquinone oxidoreductase 13kD-A subunit; anti-NDUFS6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NDUFS6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NDUFS6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human NDUFS6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUFS6 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the NDUFS6 antibody detected the NDUFS6 protein (arrow).)

Product Categories/Family for anti-NDUFS6 antibody

Antibodies; Mitochondrial Proteins

References

Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009). Martins-de-Souza, D., et al. J Neural Transm 116(3):275-289(2009). Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008). Harris, S.E., et al. BMC Genet. 8, 43 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4758792
• NCBI GeneID: 4726
• NCBI Accession #: NP_004544.1
• NCBI GenBank Nucleotide #: NM_004553.4
• UniProt Accession #: O75380
• Molecular Weight: 13,712 Da
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• NCBI Official Symbol: NDUFS6
• NCBI Official Synonym Symbols: CI-13kA; CI13KDA; CI-13kD-A
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; NADH-ubiquinone oxidoreductase 13 kDa-A subunit; NADH:ubiquinone oxidoreductase NDUFS6 subunit; complex I 13kDa sub
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
• UniProt Gene Name: NDUFS6
• UniProt Synonym Gene Names: CI-13kD-A
• UniProt Entry Name: NDUS6_HUMAN

NCBI Description

This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.[provided by RefSeq, Oct 2009]

Uniprot Description

NDUFS6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFS6 subunit family.

Protein type: EC 1.6.99.3; Mitochondrial; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity; electron carrier activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; muscle contraction; multicellular organism growth; multicellular organismal aging; fatty acid metabolic process

Disease: Mitochondrial Complex I Deficiency