S100A5 recombinant protein

S100A5 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122737

• Gene Names: S100a5; S100D9
• Purity: >97% as determined by SDS-PAGE
• Synonyms: S100A5; S100A5 Protein; Mouse; Recombinant (His Tag); Mouse S100A5 Protein (His Tag); S100D9 Protein; S100 calcium binding protein A5; S100A5 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys93

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse S100A5 (P63084)(Met 1-Lys 93) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for S100A5 recombinant protein

Background: S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.

References

Schfer,B.W. et al., 2000, J Biol Chem. 275 (39):30623-30. Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9. Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36. Heibeck TH. et al., 2009, J. Proteome Res. 8:3852-61.

NCBI and Uniprot Product Information

• NCBI GI #: 6755390
• NCBI GeneID: 20199
• NCBI Accession #: NP_035442.1
• NCBI GenBank Nucleotide #: NM_011312.2
• UniProt Accession #: P63084; O88945; P82540
• Molecular Weight: 10,812 Da
• NCBI Official Full Name: protein S100-A5
• NCBI Official Synonym Full Names: S100 calcium binding protein A5
• NCBI Official Symbol: S100a5
• NCBI Official Synonym Symbols: S100D9
• NCBI Protein Information: protein S100-A5; S100 calcium-binding protein A5
• UniProt Protein Name: Protein S100-A5
• Protein Family: Protein
• UniProt Gene Name: S100a5
• UniProt Synonym Gene Names: S100d
• UniProt Entry Name: S10A5_MOUSE

Uniprot Description

S100A5: Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites. Belongs to the S-100 family.

Cellular Component: cell soma; nucleus

Molecular Function: copper ion binding; protein homodimerization activity; zinc ion binding; metal ion binding; calcium ion binding