SDS-Page
S100A5 recombinant protein
S100A5, 1-93aa, Mouse, His tag, E Coli
Gene Names
S100a5; S100D9
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
S100A5; 1-93aa; Mouse; His tag; E Coli; S100 calcium binding protein A5; S100D9; S100A5 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 40% glycerol, 3mM DTT, 200mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAE KMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK
Sequence Length
93
Applicable Applications for S100A5 recombinant protein
SDS-PAGE
Antigen Species
Mouse
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for S100A5 recombinant protein
S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. It is expressed in very restricted regions of the adult brain. Recombinant mouse S100A5 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for S100A5 recombinant protein
References
Schafer BW., et al. (1996) Trends Biochem Sci. 21 (4):134-40. ; Schafer BW., et al. (2000) J Biol Chem. 275 (39):30623-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.4kDa (117aa), confirmed by MALDI-TOF
NCBI Official Full Name
protein S100-A5
NCBI Official Synonym Full Names
S100 calcium binding protein A5
NCBI Official Symbol
S100a5
NCBI Official Synonym Symbols
S100D9
NCBI Protein Information
protein S100-A5
UniProt Protein Name
Protein S100-A5
Protein Family
UniProt Gene Name
S100a5
UniProt Synonym Gene Names
S100d
Uniprot Description
Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites ().
Research Articles on S100A5
Similar Products
Product Notes
The S100A5 s100a5 (Catalog #AAA204748) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's S100A5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the S100A5 s100a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAE KMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK. It is sometimes possible for the material contained within the vial of "S100A5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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