RTN4 recombinant protein

RTN4 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121248

• Gene Names: RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• Purity: >70% as determined by SDS-PAGE
• Synonyms: RTN4; RTN4 Protein; Human; Recombinant (GST Tag); Human RTN4/NOGO-A Protein (GST Tag); ASY Protein; DGU Protein; DKFZp781L1143 Protein; HIGM4 Protein; Nbla00271 Protein; Nbla10545 Protein; NI220/250 Protein; NOGO Protein; NOGO-A Protein; Nogo-B Protein; Nogo-C Protein; NOGOC Protein; NSP Protein; NSP-CL Protein; RTN-X Protein; RTN4-A Protein; RTN4-B1 Protein; RTN4-B2 Protein; RTN4-C Protein; UDG Protein; UNG1 Protein; UNG15 Protein; UNG2 Protein; reticulon 4; RTN4 recombinant protein

• Host: E Coli
• Purity/Purification: >70% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 1mM DTT, 0.2mM GSH, pH7.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val185

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human RTN4 (NP_065393.1) N-terminal fragment (Met 1-Val 185) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RTN4 recombinant protein

Background: Reticulon-4, also known as Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5 and RTN4, is a multi-pass membrane protein that contains one reticulon domain. Isoform 1 of RTN4 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 of RTN4 is widely expressed except for the liver. Isoform 3 of RTN4 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 of RTN4 is testis-specific. Reticulon-4/RTN4 is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon-4/RTN4 regulates neurite fasciculation, branching and extension in the developing nervous system. Reticulon-4/RTN4 is involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 of RTN4 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 of RTN4 inhibit BACE1 activity and amyloid precursor protein processing.

References

Yang J., et al., 2000, Cytogenet. Cell Genet. 88:101-102. Prinjha R., et al., 2000, Nature 403: 383-384. Tagami S., et al., 2000, Oncogene 19: 5736-5746. Murayama K.S., et al., 2006, Eur. J. Neurosci. 24: 1237-1244. Daub H., et al., 2008, Mol. Cell 31:438-448. Choudhary C., et al., 2009, Science 325: 834-840.

NCBI and Uniprot Product Information

• NCBI GI #: 24431935
• NCBI GeneID: 57142
• NCBI Accession #: NP_065393.1
• NCBI GenBank Nucleotide #: NM_020532.4
• UniProt Accession #: Q9NQC3; O94962; Q7L7Q5; Q7L7Q6; Q7L7Q8; Q8IUA4; Q96B16; Q9BXG5; Q9H212; Q9H3I3; Q9UQ42; Q9Y293
• Molecular Weight: 108,450 Da
• NCBI Official Full Name: reticulon-4 isoform A
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: RTN4
• NCBI Official Synonym Symbols: ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• NCBI Protein Information: reticulon-4; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog
• UniProt Protein Name: Reticulon-4
• Protein Family: Reticulon
• UniProt Gene Name: RTN4
• UniProt Synonym Gene Names: KIAA0886; NOGO; Nogo protein; NSP
• UniProt Entry Name: RTN4_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Inhibitor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular

Molecular Function: protein binding

Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; apoptosis; cerebral cortex radial glia guided migration; axonal fasciculation; negative regulation of axonogenesis; negative regulation of cell growth; angiogenesis; negative regulation of axon extension; regulation of axonogenesis; regulation of cell migration