Rabbit RTN4 / Nogo Polyclonal Antibody | anti-RTN4 antibody

Rabbit Polyclonal to Human RTN4 / Nogo

Cat. Num. AAA242170

• Gene Names: RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: RTN4 / Nogo; Polyclonal Antibody; Rabbit Polyclonal to Human RTN4 / Nogo; Anti-RTN4 / Nogo Antibody (N-Terminus) IHC-plus; RTN4; Human NogoA; My043 protein; Neurite outgrowth inhibitor; NOGOC; NSP-CL; Nogo protein; KIAA0886; Reticulon 5; Reticulon-4; Reticulon-5; Reticulon 4; RTN4-B1; RTN-X; RTN4-A; RTN4-B2; RTN4-C; Nbla00271; Neurite growth inhibitor 220; NI220/250; Nogo; Human RTN4; anti-RTN4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 23 amino acid peptide from near the amino terminus of human NogoA.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1192

• Applicable Applications for anti-RTN4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (3 ug/ml), WB (0.5 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RTN4 / Nogo.
• Immunogen Type: Synthetic peptide
• Immunogen: RTN4 / Nogo antibody was raised against synthetic peptide from human RTN4 / Nogo.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 ug/ml.)

Related Product Information for anti-RTN4 antibody

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates.

Product Categories/Family for anti-RTN4 antibody

Family: Reticulon

NCBI and Uniprot Product Information

• NCBI GI #: 24431935
• NCBI GeneID: 57142
• NCBI Accession #: NP_065393.1
• NCBI GenBank Nucleotide #: NM_020532.4
• UniProt Accession #: Q9NQC3; O94962; Q7L7Q5; Q7L7Q6; Q7L7Q8; Q8IUA4; Q96B16; Q9BXG5; Q9H212; Q9H3I3; Q9UQ42; Q9Y293
• Molecular Weight: 108,450 Da
• NCBI Official Full Name: reticulon-4 isoform A
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: RTN4
• NCBI Official Synonym Symbols: ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• NCBI Protein Information: reticulon-4; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog
• UniProt Protein Name: Reticulon-4
• Protein Family: Reticulon
• UniProt Gene Name: RTN4
• UniProt Synonym Gene Names: KIAA0886; NOGO; Nogo protein; NSP
• UniProt Entry Name: RTN4_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Inhibitor; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular

Molecular Function: protein binding

Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; cerebral cortex radial glia guided migration; apoptosis; axonal fasciculation; negative regulation of axonogenesis; angiogenesis; negative regulation of axon extension; negative regulation of cell growth; regulation of axonogenesis; regulation of cell migration