RTCA recombinant protein

RTCA, 1-366aa, Human, His tag, E Coli

Cat. Num. AAA205764

• Gene Names: RTCA; RPC; RTC1; RTCD1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RTCA; 1-366aa; Human; His tag; E Coli; RNA 3-terminal phosphate cyclase isoform b; RNA 3'-terminal phosphate cyclase; RPC; RTC1; RTCD1; RTCA recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL
• Sequence Length: 366

• Applicable Applications for RTCA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RTCA recombinant protein

RTCA catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RTCA recombinant protein

Epigenomics (Transcription & Translation)

References

Genschik P., Billy E., et al. (1997) EMBO J. 16:2955-2967

NCBI and Uniprot Product Information

• NCBI GI #: 4506589
• NCBI GeneID: 8634
• NCBI Accession #: NP_003720
• NCBI GenBank Nucleotide #: NM_003729.3
• UniProt Accession #: O00442; Q5VVL5; Q5VVL6; Q96E99
• Molecular Weight: 41.7 kDa (389aa) confirmed by MALDI-TOF
• NCBI Official Full Name: RNA 3'-terminal phosphate cyclase isoform b
• NCBI Official Synonym Full Names: RNA 3'-terminal phosphate cyclase
• NCBI Official Symbol: RTCA
• NCBI Official Synonym Symbols: RPC; RTC1; RTCD1
• NCBI Protein Information: RNA 3'-terminal phosphate cyclase
• UniProt Protein Name: RNA 3'-terminal phosphate cyclase
• Protein Family: RNA 3'-terminal phosphate cyclase
• UniProt Gene Name: RTCA
• UniProt Synonym Gene Names: RPC; RPC1; RTC1; RTCD1; RNA cyclase; RNA-3'-phosphate cyclase; RTC domain-containing protein 1

NCBI Description

This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.