Rat Aquaporin 2 ELISA Kit | AQP-2 elisa kit

Rat Aquaporin 2 ELISA Kit

Cat. Num. AAA765367

• Gene Names: AQP2; AQP-CD; WCH-CD
• Reactivity: Rat
• Synonyms: Aquaporin 2; Rat Aquaporin 2 ELISA Kit; AQP-2/Aquaporin-2/Water channel protein for renal collecting duct/WCH-CD/Collecting duct water channel protein/ADH water channel/Aquaporin-CD (AQP-CD); AQP-2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of AQP-2. No significant cross-reactivity or interference between AQP-2 and analogues was observed.
• Sequence Length: 271

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.563-100ng/ml
• Sensitivity: <0.938ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AQP-2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AQP-2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AQP-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AQP-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AQP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AQP-2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5052748
• NCBI GeneID: 359
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: aquaporin 2
• NCBI Official Synonym Full Names: aquaporin 2
• NCBI Official Symbol: AQP2
• NCBI Official Synonym Symbols: AQP-CD; WCH-CD
• NCBI Protein Information: aquaporin-2
• UniProt Protein Name: Aquaporin-2
• UniProt Gene Name: AQP2
• UniProt Synonym Gene Names: AQP-2; AQP-CD
• UniProt Entry Name: AQP2_HUMAN

NCBI Description

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

Uniprot Description

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: apical plasma membrane; basolateral plasma membrane; Golgi apparatus; integral to membrane; membrane; plasma membrane; recycling endosome

Molecular Function: glycerol channel activity; glycerol transmembrane transporter activity; water channel activity; water transporter activity

Biological Process: cellular response to water deprivation; excretion; glycerol transport; renal water homeostasis; renal water transport; transmembrane transport; water transport

Disease: Diabetes Insipidus, Nephrogenic, Autosomal