RPL22 recombinant protein

RPL22, 1-128aa, Human, His tag, E Coli

Cat. Num. AAA205213

• Gene Names: RPL22; EAP; L22; HBP15; HBP15/L22
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RPL22; 1-128aa; Human; His tag; E Coli; 60S ribosomal protein L22 proprotein; EAP; HBP15; HBP15/L22; L22; RPL22 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPVKKL VVKGGKKKKQ VLKFTLDCTH PVEDGIMDAA NFEQFLQERI KVNGKAGNLG GGVVTIERSK SKITVTSEVP FSKRYLKYLT KKYLKKNNLR DWLRVVANSK ESYELRYFQI NQDEEEEEDE D
• Sequence Length: 128

• Applicable Applications for RPL22 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RPL22 recombinant protein

RPL22 is a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. Recombinant human RPL22 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RPL22 recombinant protein

Epigenomics

References

Fok V, Mitton-Fry RM, et al. (2006). RNA. 12 (5):872-82. ; Shu-Nu C, Lin CH, et al. (2000). FEBS Lett. 484 (1):22-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 4506613
• NCBI GeneID: 6146
• NCBI Accession #: NP_000974
• NCBI GenBank Nucleotide #: NM_000983.3
• UniProt Accession #: P35268; Q6IBD1; B2R495
• Molecular Weight: 17 kDa (151aa), confirmed by MALDI-TOF
• NCBI Official Full Name: 60S ribosomal protein L22
• NCBI Official Synonym Full Names: ribosomal protein L22
• NCBI Official Symbol: RPL22
• NCBI Official Synonym Symbols: EAP; L22; HBP15; HBP15/L22
• NCBI Protein Information: 60S ribosomal protein L22
• UniProt Protein Name: 60S ribosomal protein L22
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL22
• UniProt Synonym Gene Names: EAP

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousBinds to Epstein-Barr virus small RNAs and to heparin.