Rabbit RTCD1 Polyclonal Antibody | anti-RTCD1 antibody

RTCD1 Polyclonal Antibody

Cat. Num. AAA3006191

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RTCD1; Polyclonal Antibody; RTCD1 Polyclonal Antibody; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1; RTCA; RPC; RPC1; RTC1; anti-RTCD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RTCD1 polyclonal antibody detects endogenous levels of RTCD1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 366

• Applicable Applications for anti-RTCD1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 321-366 of Human RTCD1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RTCD1 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RTCD1 pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-RTCD1 antibody

RTCD1 catalyzes the conversion of 3'-phosphate to a 2', 3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 4506589
• NCBI Accession #: NP_003720
• NCBI GenBank Nucleotide #: NP_003720.1
• UniProt Accession #: O00442
• Molecular Weight: ~ 39kDa
• NCBI Official Full Name: RNA 3'-terminal phosphate cyclase isoform b
• UniProt Protein Name: RNA 3'-terminal phosphate cyclase
• UniProt Gene Name: RTCA
• UniProt Synonym Gene Names: RPC; RPC1; RTC1; RTCD1; RNA cyclase; RNA-3'-phosphate cyclase; RTC domain-containing protein 1

Uniprot Description

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.