Mouse peptidyl arginine deiminase, type IV ELISA Kit | PADI4 elisa kit

Mouse Protein-arginine deiminase type-4, PADI4 ELISA Kit

Cat. Num. AAA906945

• Gene Names: Padi4; Pad4; Pdi4
• Reactivity: Mouse
• Synonyms: peptidyl arginine deiminase; type IV; Mouse Protein-arginine deiminase type-4; PADI4 ELISA Kit; Mouse Protein-arginine deiminase type-4 (PADI4) ELISA kit; PAD; PAD4; PADI5; PDI4; PDI5; HL-60 PAD; PADI-H protein; type V; protein-arginine deiminase; PADI4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PADI4. No significant cross-reactivity or interference between mouse PADI4 and analogues was observed.
• Sequence Length: 666

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 4.7 pg/ml-300 pg/ml
• Sensitivity: < 1.17 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PADI4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PADI4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 156938252
• NCBI GeneID: 18602
• NCBI Accession #: NP_035191.2
• NCBI GenBank Nucleotide #: NM_011061.2
• UniProt Accession #: Q9Z183; Q75WC7; A2AMU3
• Molecular Weight: 74,415 Da
• NCBI Official Full Name: protein-arginine deiminase type-4
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type IV
• NCBI Official Symbol: Padi4
• NCBI Official Synonym Symbols: Pad4; Pdi4
• NCBI Protein Information: protein-arginine deiminase type-4; PAD type IV; peptidylarginine deiminase IV; protein-arginine deiminase type IV
• UniProt Protein Name: Protein-arginine deiminase type-4
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: Padi4
• UniProt Synonym Gene Names: Pdi4
• UniProt Entry Name: PADI4_MOUSE

Uniprot Description

PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.

Protein type: Hydrolase; EC 3.5.3.15; Nuclear receptor co-regulator

Cellular Component: cytoplasm; nucleus

Molecular Function: arginine deiminase activity; hydrolase activity; metal ion binding; calcium ion binding; protein-arginine deiminase activity

Biological Process: chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; transcription, DNA-dependent; immune system process; stem cell maintenance; innate immune response; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine