Western Blot (WB)
(Western blot analysis of lysates from MDA-MB-453,NCI-H292,K562 cell line (from left to right), using ICT1 Antibody (C-term) (MBS9231683). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Rabbit anti-Human ICT1 Polyclonal Antibody | anti-ICT1 antibody
ICT1 Antibody (C-term)
IHC-P~~1:25
Western Blot (WB)
(Western blot analysis of lysates from MDA-MB-453,NCI-H292,K562 cell line (from left to right), using ICT1 Antibody (C-term) (MBS9231683). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from MDA-MB-453,NCI-H292,K562 cell line (from left to right), using ICT1 Antibody (C-term) (MBS9231683). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody (C-term) (MBS9231683) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using ICT1 Antibody (C-term) (MBS9231683) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody (C-term) (MBS9231683). It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. skin section using ICT1 Antibody (C-term) (MBS9231683). It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Background: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
Uniprot Description
ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.
Protein type: Mitochondrial; EC 3.1.1.29
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial large ribosomal subunit
Molecular Function: aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific
Biological Process: mitochondrial translation; organelle organization and biogenesis
Research Articles on ICT1
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Product Notes
The ICT1 ict1 (Catalog #AAA9231683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ICT1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB~~1:1000 IHC-P~~1:25. Researchers should empirically determine the suitability of the ICT1 ict1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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