p53R2 ELISA Pair Set | RRM2B elisa pair set

p53R2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118445

• Synonyms: p53R2; p53R2 Matched ELISA Antibody Pair Set; Human; Human RRM2B/P53R2 ELISA Pair Set; ribonucleotide reductase M2 B (TP53 inducible); MTDPS8A Matched ELISA Antibody Pair Set; MTDPS8B Matched ELISA Antibody Pair Set; P53R2 Matched ELISA Antibody Pair Set; RRM2B elisa pair set

• Specificity: Quantitative determination of Human p53R2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 46.88-3000pg/mL
• Sensitivity: 47pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-RRM2B monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL mouse anti-RRM2B monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 63 ng of recombinant RRM2B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for RRM2B elisa pair set

Ribonucleoside reductase subunit M2B, also known as RRM2B or p53R2, is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. RRM2B is a phosphorylated protein. It is hypothesized that RRM2B activity can be regulated at the posttranslational level in response to DNA damage. RRM2B has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer types. However, the contribution of RRM2B to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of RRM2B posttranslationally. Besides, a defect in RRM2B can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic.

This p53R2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human p53R2. It contains Human p53R2 capture antibody, Human p53R2 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human p53R2 protein.

References

Bourdon A, et al. (2007) Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2), causes severe mitochondrial DNA depletion. Nature Genetics. 39: 776-80.Tyynismaa H, et al. (2009) A Heterozygous Truncating Mutation in RRM2B Causes Autosomal-Dominant Progressive External Ophthalmoplegia with Multiple mtDNA Deletions. AJHG. 85 (2) : 290-5.Shaibani A, et al. (2009) Mitochondrial neurogastrointestinal encephalopathy due to mutations in RRM2B. Arch Neurol.66 (8): 1028-32.