RRM2B / P53R2 recombinant protein

Recombinant Human RRM2B / P53R2 Protein (His tag)

Cat. Num. AAA2545383

• Gene Names: RRM2B; P53R2; MTDPS8A; MTDPS8B
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: RRM2B / P53R2; Recombinant Human RRM2B / P53R2 Protein (His tag); DKFZp686M05248; MGC102856; MGC42116; MTDPS8A; MTDPS8B; P53R2; RRM2B / P53R2 recombinant protein

• Host: E Coli
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 30% glycerol, pH 8.5
• Sequence Length: 423

• Application Notes: The recombinant human RRM2B consisting of 366 amino acids and has a calculated molecular mass of 42.6 kDa. It migrates as a 43 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for RRM2B / P53R2 recombinant protein

Background: Ribonucleoside reductase subunit M2B, also known as RRM2B or p53R2, is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. RRM2B is a phosphorylated protein. It is hypothesized that RRM2B activity can be regulated at the posttranslational level in response to DNA damage. RRM2B has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer type s. However, the contribution of RRM2B to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of RRM2B posttranslationally. In addition, a defect in RRM2B can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic. 

Description: A DNA sequence encoding the human RRM2B (Q7LG56-1) (Met 1-Phe 351) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 289177074
• NCBI GeneID: 50484
• NCBI Accession #: NP_001165948.1
• NCBI GenBank Nucleotide #: NM_001172477.1
• UniProt Accession #: Q7LG56; Q17R22; Q75PQ6; Q75PQ7; Q75PY8; Q75PY9; Q86YE3; Q9NPD6; Q9NTD8; Q9NUW3; B4E2N4
• Molecular Weight: 48,787 Da
• NCBI Official Full Name: ribonucleoside-diphosphate reductase subunit M2 B isoform 2
• NCBI Official Synonym Full Names: ribonucleotide reductase regulatory TP53 inducible subunit M2B
• NCBI Official Symbol: RRM2B
• NCBI Official Synonym Symbols: P53R2; MTDPS8A; MTDPS8B
• NCBI Protein Information: ribonucleoside-diphosphate reductase subunit M2 B
• UniProt Protein Name: Ribonucleoside-diphosphate reductase subunit M2 B
• UniProt Gene Name: RRM2B
• UniProt Synonym Gene Names: P53R2; p53R2

NCBI Description

This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]

Uniprot Description

Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.