Raptor Blocking Peptide | Rptor blocking peptide

Raptor Peptide

Cat. Num. AAA152447

• Gene Names: Rptor; Rap; Raptor; mKIAA1303; 4932417H02Rik
• Applications: Blocking (BL)
• Synonyms: Raptor; Raptor Peptide; Rap; mKIAA1303; 4932417H02Rik; Regulatory-associated protein of mTOR; regulatory associated protein of MTOR; complex 1; Rptor blocking peptide

• Form/Format: Liquid
• Concentration: 200 ug/mL (varies by lot)
• Sequence Length: 1335

• Applicable Applications for Rptor blocking peptide: Blocking (BL)
• Application Notes: Raptor peptide is used for blocking the activity of the Raptor antibody.
• Species: Human
• Buffer: PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide
• Location: 13 amino acids near the amino terminus of human Raptor.
• Preparation and Storage: Store Raptor peptide at -20 degree C, stable for one year.

NCBI and Uniprot Product Information

• NCBI GI #: 46577497
• NCBI GeneID: 74370
• UniProt Accession #: Q8K4Q0; Q8C9W9; Q8CBY4; Q8CDY8; Q9D4H3
• Molecular Weight: 33,651 Da
• NCBI Official Full Name: Regulatory-associated protein of mTOR
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: Rptor
• NCBI Official Synonym Symbols: Rap; Raptor; mKIAA1303; 4932417H02Rik
• NCBI Protein Information: regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: Rptor
• UniProt Synonym Gene Names: Raptor; Raptor
• UniProt Entry Name: RPTOR_MOUSE

NCBI Description

This gene encodes a subunit of mammalian target of rapamycin complex 1 (mTORC1), a component of the mTOR signaling pathway, which regulates cell growth in response to nutrient and energy levels. The encoded protein may regulate the assembly, localization, and substrate binding of the mTORC1 complex. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; cell soma; intracellular membrane-bound organelle; lysosome; lysosomal membrane; cytoplasm; dendrite

Molecular Function: protein binding; protein complex binding; protein kinase binding

Biological Process: cellular response to nutrient levels; TOR signaling pathway; regulation of phosphorylation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; cell growth; regulation of cell size; positive regulation of TOR signaling pathway