Rabbit anti-Mouse Raptor Polyclonal Antibody | anti-Rptor antibody

Raptor Antibody

Cat. Num. AAA151576

• Gene Names: Rptor; Rap; Raptor; mKIAA1303; 4932417H02Rik
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Raptor Antibody is affinity chromatography purified via peptide column.
• Synonyms: Raptor; Polyclonal Antibody; Raptor Antibody; Rap; mKIAA1303; 4932417H02Rik; Regulatory-associated protein of mTOR; regulatory associated protein of MTOR; complex 1; anti-Rptor antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Raptor has multiple isoforms that may also be recognized by antibody.
• Purity/Purification: Raptor Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1335

• Applicable Applications for anti-Rptor antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Raptor (CT) polyclonal antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of mouse Raptor.
• Buffer: Raptor Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Raptor antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of Raptor in L1210 cells with Raptor antibody at 10 μg/mL.)

Related Product Information for anti-Rptor antibody

Raptor Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 46577497
• NCBI GeneID: 74370
• UniProt Accession #: Q8K4Q0; Q8C9W9; Q8CBY4; Q8CDY8; Q9D4H3
• Molecular Weight: 33,651 Da
• NCBI Official Full Name: Regulatory-associated protein of mTOR
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: Rptor
• NCBI Official Synonym Symbols: Rap; Raptor; mKIAA1303; 4932417H02Rik
• NCBI Protein Information: regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: Rptor
• UniProt Synonym Gene Names: Raptor; Raptor
• UniProt Entry Name: RPTOR_MOUSE

NCBI Description

This gene encodes a subunit of mammalian target of rapamycin complex 1 (mTORC1), a component of the mTOR signaling pathway, which regulates cell growth in response to nutrient and energy levels. The encoded protein may regulate the assembly, localization, and substrate binding of the mTORC1 complex. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: nucleoplasm; cell soma; intracellular membrane-bound organelle; lysosomal membrane; lysosome; cytoplasm; dendrite

Molecular Function: protein binding; protein complex binding; protein kinase binding

Biological Process: cellular response to nutrient levels; TOR signaling pathway; regulation of phosphorylation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; cell growth; regulation of cell size; positive regulation of TOR signaling pathway