Western Blot (WB)
(POLE3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).)
Rabbit anti-Human POLE3 Polyclonal Antibody | anti-POLE3 antibody
POLE3, NT (DNA Polymerase epsilon Subunit 3, DNA Polymerase II Subunit 3, DNA Polymerase epsilon Subunit p17, Chromatin Accessibility Complex 17kD Protein, HuCHRAC17, CHRAC-17, Arsenic-transactivated Protein, AsTP, CHRAC17) (HRP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(POLE3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).)
Western Blot (WB)
(POLE3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).)
Flow Cytometry (FC/FACS)
(POLE3 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(POLE3 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
Uniprot Description
POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Protein type: EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: epsilon DNA polymerase complex; nucleus
Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding; DNA-directed DNA polymerase activity
Biological Process: DNA replication
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Product Notes
The POLE3 pole3 (Catalog #AAA6494103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLE3, NT (DNA Polymerase epsilon Subunit 3, DNA Polymerase II Subunit 3, DNA Polymerase epsilon Subunit p17, Chromatin Accessibility Complex 17kD Protein, HuCHRAC17, CHRAC-17, Arsenic-transactivated Protein, AsTP, CHRAC17) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the POLE3 pole3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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