SDS-Page
Ribophorin I (RPN1) Recombinant Protein | RPN1 recombinant protein
Recombinant Ribophorin I (RPN1)
Gene Names
RPN1; OST1; RBPH1
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Ribophorin I (RPN1); Recombinant Ribophorin I (RPN1); RPNI; OST1; RBPH1; Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1; Dolichyl-diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit; RPN1 recombinant protein
Host
Host: E Coli
Source: Prokaryotic Expression
Source: Prokaryotic Expression
Purity/Purification
> 90%
Form/Format
Freeze-Dried Powder; 20mM Tris, 150mM NaCl, pH8.0, Containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Ser272~Lys517
Sequence Length
607
Applicable Applications for RPN1 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Species
Human
Tag
N-terminal His and GST-Tag
Isoelectric Point
6.4
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Subcellular Location
Endoplasmic Reticulum Lumen
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted MW: 58.3kDa
Accurate MW: 58kDa
Accurate MW: 58kDa
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
NCBI Official Synonym Full Names
ribophorin I
NCBI Official Symbol
RPN1
NCBI Official Synonym Symbols
OST1; RBPH1
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Protein Family
UniProt Gene Name
RPN1
UniProt Synonym Gene Names
RPN-I
UniProt Entry Name
RPN1_HUMAN
NCBI Description
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]
Research Articles on RPN1
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Product Notes
The RPN1 rpn1 (Catalog #AAA2105419) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic Expression and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser272~Lys517. AAA Biotech's Ribophorin I (RPN1) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the RPN1 rpn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribophorin I (RPN1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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