Mouse anti-Human HCLS1 Monoclonal Antibody | anti-HCLS1 antibody

Mouse Monoclonal [clone 2A8] (IgG1,k) to Human HCLS1

Cat. Num. AAA246336

• Gene Names: HCLS1; HS1; p75; CTTNL; lckBP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HCLS1; Monoclonal Antibody; Mouse Monoclonal [clone 2A8] (IgG1; k) to Human HCLS1; Anti-HCLS1 Antibody (clone 2A8) IHC-plus; CTTNL; LckBP1; p75; HS1; Human HCLS1; anti-HCLS1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A8
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 486

• Applicable Applications for anti-HCLS1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control K-562. ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: HCLS1 (AAH16758, 266 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: HCLS1 antibody was raised against hCLS1 (AAH16758, 266 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-HCLS1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HCLS1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HCLS1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(HCLS1 monoclonal antibody, clone 2A8 Western blot of HCLS1 expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged HCLS1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 167234422
• NCBI GeneID: 3059
• NCBI Accession #: NP_005326.2
• NCBI GenBank Nucleotide #: NM_005335.5
• UniProt Accession #: P14317; Q53Y93; Q6IBK9; Q9UDK0; B4DQ69
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: hematopoietic lineage cell-specific protein isoform 1
• NCBI Official Synonym Full Names: hematopoietic cell-specific Lyn substrate 1
• NCBI Official Symbol: HCLS1
• NCBI Official Synonym Symbols: HS1; p75; CTTNL; lckBP1
• NCBI Protein Information: hematopoietic lineage cell-specific protein
• UniProt Protein Name: Hematopoietic lineage cell-specific protein
• Protein Family: HCLS1-binding protein
• UniProt Gene Name: HCLS1
• UniProt Synonym Gene Names: HS1
• UniProt Entry Name: HCLS1_HUMAN

Uniprot Description

HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: transcription factor complex; mitochondrion; membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein complex binding; actin binding; SH3 domain binding; protein kinase binding

Biological Process: positive regulation of granulocyte differentiation; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; regulation of actin filament polymerization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of STAT protein; regulation of transcription, DNA-dependent; actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of macrophage differentiation