Goat MURF1 / IRF Polyclonal Antibody | anti-MURF1 antibody

Goat Polyclonal to Human MURF1 / IRF

Cat. Num. AAA241643

• Gene Names: TRIM63; IRF; SMRZ; MURF1; MURF2; RNF28
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Bat, Bovine, Dog, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MURF1 / IRF; Polyclonal Antibody; Goat Polyclonal to Human MURF1 / IRF; Anti-MURF1 / IRF Antibody (Internal) IHC-plus; TRIM63; Iris RING finger protein; MURF-1; MURF1; MuRF2; IRF; RING finger protein 28; RNF28; Tripartite motif-containing 63; Tripartite motif containing 63; SMRZ; Human MURF1; anti-MURF1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Bat, Bovine, Dog, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TRIM63 / MURF1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 353

• Applicable Applications for anti-MURF1 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (1.25 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QQYQEQLDKSTKLVE from an internal region of human TRIM63 / MURF1 (NP_115977.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Bovine, Elephant, Panda, Horse (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: MURF1 / IRF antibody was raised against synthetic peptide C-QQYQEQLDKSTKLVE from an internal region of human TRIM63 / MURF1 (NP_115977.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Bovine, Elephant, Panda, Horse (93%); Rabbit, Platypus (87%); Mouse, Rat, Hamster, Opossum (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TRIM63 / MURF1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.)

Product Categories/Family for anti-MURF1 antibody

Family: Tripartite Motif

NCBI and Uniprot Product Information

• NCBI GI #: 19924163
• NCBI GeneID: 84676
• NCBI Accession #: NP_115977.2
• NCBI GenBank Nucleotide #: NM_032588.3
• UniProt Accession #: Q969Q1; Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95
• Molecular Weight: 36,950 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM63
• NCBI Official Synonym Full Names: tripartite motif containing 63, E3 ubiquitin protein ligase
• NCBI Official Symbol: TRIM63
• NCBI Official Synonym Symbols: IRF; SMRZ; MURF1; MURF2; RNF28
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM63
• UniProt Gene Name: TRIM63
• UniProt Synonym Gene Names: IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1
• UniProt Entry Name: TRI63_HUMAN

NCBI Description

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

Uniprot Description

MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: microtubule; cytoplasm; M band; nucleus; Z disc

Molecular Function: signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction