Typical Testing Data/Standard Curve (for reference only)
Human Nuclear receptor ROR-gamma ELISA Kit | RORC elisa kit
Human Nuclear receptor ROR-gamma ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RORG, During the reaction, RORG in the sample or standard competes with a fixed amount of biotin-labeled RORG for sites on a pre-coated Monoclonal antibody specific to RORG. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RORG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
RORC: Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T-helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Nuclear receptor; Transcription factor
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleoplasm; nucleus
Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; oxysterol binding; protein binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; zinc ion binding
Biological Process: circadian regulation of gene expression; gene expression; intracellular receptor-mediated signaling pathway; lymph node development; Peyer's patch development; positive regulation of circadian rhythm; positive regulation of transcription, DNA-dependent; regulation of fat cell differentiation; regulation of steroid metabolic process; steroid hormone mediated signaling; T-helper cell differentiation; transcription initiation from RNA polymerase II promoter; xenobiotic metabolic process
Disease: Immunodeficiency 42
Research Articles on RORC
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Product Notes
The Human RORC rorc (Catalog #AAA2884310) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884310 ELISA Kit recognizes Human RORC. It is sometimes possible for the material contained within the vial of "Nuclear receptor ROR-gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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