Rat Ras GTPase-Activating Protein SynGAP (SYNGAP1) ELISA Kit | SYNGAP1 elisa kit

Rat Ras GTPase-Activating Protein SynGAP (SYNGAP1) ELISA Kit

Cat. Num. AAA9711757

• Gene Names: Syngap1; Syngap
• Reactivity: Rat
• Synonyms: Ras GTPase-Activating Protein SynGAP (SYNGAP1); Rat Ras GTPase-Activating Protein SynGAP (SYNGAP1) ELISA Kit; SYNGAP1; DASS-97D12.6; DKFZp761G1421; KIAA1938; MRD5; RASA1; RASA5; SYNGAP; Ras GTPase-activating protein SynGAP; neuronal RasGAP; synaptic Ras GTPase activating protein 1; synaptic Ras GTPase activating protei; SYNGAP1 elisa kit

• Reactivity: Rat
• Specificity: Rat Ras GTPase-activating protein SynGAP (SYNGAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SYNGAP1. No significant cross-reactivity or interference between Rat SYNGAP1 and analogues was observed.
• Sequence Length: 1308

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Ras GTPase-activating protein SynGAP (SYNGAP1) Standard Curve.)

Related Product Information for SYNGAP1 elisa kit

Description: This Rat Ras GTPase-activating protein SynGAP (SYNGAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNGAP1 in samples. An antibody specific for SYNGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYNGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: SYNGAP1 is a ras GTPase-activating protein that is critical for cognition and synapse function. Mutations in humans cause mental retardation. Several mutations in the SYNGAP1 gene were identified as the cause of mental retardation. Mental retardation is sometimes associated with syndromes of other defects caused by the same gene, but SYNGAP1-associated mental retardation is not; it is therefore called non-syndromic mental retardation. Since neither of the parents of children with this condition have the mutation, this means it was a sporadic mutation that occurred during division of the parents' gametes (meiosis) or fertilization of the egg. It is a dominant mutation, which means that the individual will be retarded if only one allele is mutated.

NCBI and Uniprot Product Information

• NCBI GI #: 150421677
• NCBI GeneID: 192117
• UniProt Accession #: Q9QUH6
• NCBI Official Full Name: Ras/Rap GTPase-activating protein SynGAP
• NCBI Official Synonym Full Names: synaptic Ras GTPase activating protein 1
• NCBI Official Symbol: Syngap1
• NCBI Official Synonym Symbols: Syngap
• NCBI Protein Information: ras/Rap GTPase-activating protein SynGAP
• UniProt Protein Name: Ras/Rap GTPase-activating protein SynGAP
• Protein Family: Ras/Rap GTPase-activating protein
• UniProt Gene Name: Syngap1
• UniProt Synonym Gene Names: Synaptic Ras-GAP 1
• UniProt Entry Name: SYGP1_RAT

NCBI Description

This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations in mice result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]