Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keyhole limpet hemocyanin IgG antibody (KLH-Ab-IgG) Standard Curve.)
Rat Keyhole Limpet Hemocyanin IgG (KLH-Ab-IgG) ELISA Kit | KLH-Ab-IgG elisa kit
Rat Keyhole Limpet Hemocyanin IgG Antibody (KLH-Ab-IgG) ELISA Kit
Reactivity
Rat
Synonyms
Keyhole Limpet Hemocyanin IgG (KLH-Ab-IgG); Rat Keyhole Limpet Hemocyanin IgG Antibody (KLH-Ab-IgG) ELISA Kit; KLH-Ab-IgG elisa kit
Reactivity
Rat
Specificity
Rat Keyhole limpet hemocyanin IgG antibody (KLH-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KLH-Ab-IgG. No significant cross-reactivity or interference between Rat KLH-Ab-IgG and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keyhole limpet hemocyanin IgG antibody (KLH-Ab-IgG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keyhole limpet hemocyanin IgG antibody (KLH-Ab-IgG) Standard Curve.)
Related Product Information for KLH-Ab-IgG elisa kit
Description: This Rat Keyhole limpet hemocyanin IgG antibody (KLH-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate KLH-Ab-IgG in samples. An antibody specific for KLH-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLH-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLH-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLH-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein found in the hemolymph of the giant keyhole limpet, Megathura crenulata, that lives off the coast of California from Monterey Bay to Isla Asuncion off Baja California. Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.
Background: Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein found in the hemolymph of the giant keyhole limpet, Megathura crenulata, that lives off the coast of California from Monterey Bay to Isla Asuncion off Baja California. Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.
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Product Notes
The Rat KLH-Ab-IgG (Catalog #AAA9712673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712673 ELISA Kit recognizes Rat KLH-Ab-IgG. It is sometimes possible for the material contained within the vial of "Keyhole Limpet Hemocyanin IgG (KLH-Ab-IgG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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