Human Nuclear receptor ROR-gamma ELISA Kit | RORC elisa kit

Human Nuclear receptor ROR-gamma ELISA Kit

Cat. Num. AAA760662

• Gene Names: RORC; TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
• Reactivity: Human
• Synonyms: Nuclear receptor ROR-gamma; Human Nuclear receptor ROR-gamma ELISA Kit; RORC/ROR gamma/NR1F3/RZRG/MGC129539/nuclear receptor ROR-gamma/Nuclear receptor RZR-gamma/Nuclear receptor subfamily 1 group F member 3/RAR-related orphan receptor C/RAR-related orphan receptor gamma/retinoic acid-binding receptor gamma/retinoid-related orphan receptor gamma/Retinoid-related orphan receptor-gamma/RORGMGC129539/RZRGRZR-GAMMA/TOR; RORC elisa kit

• Reactivity: Human
• Sequence Length: 497

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RORC elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RORC. During the reaction, RORC in the sample or standard competes with a fixed amount of biotin-labeled RORC for sites on a pre-coated Monoclonal antibody specific to RORC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RORC in the samples is then determined by comparing the OD. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 48255918
• NCBI GeneID: 6097
• NCBI Accession #: NP_001001523.1
• NCBI GenBank Nucleotide #: NM_001001523.1
• Molecular Weight: 55,813 Da
• NCBI Official Full Name: nuclear receptor ROR-gamma isoform b
• NCBI Official Synonym Full Names: RAR related orphan receptor C
• NCBI Official Symbol: RORC
• NCBI Official Synonym Symbols: TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
• NCBI Protein Information: nuclear receptor ROR-gamma
• UniProt Protein Name: Nuclear receptor ROR-gamma
• Protein Family: Nuclear receptor
• UniProt Gene Name: RORC
• UniProt Synonym Gene Names: NR1F3; RORG; RZRG
• UniProt Entry Name: RORG_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RORC: Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T-helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: intracellular membrane-bound organelle; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; oxysterol binding; protein binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; zinc ion binding

Biological Process: circadian regulation of gene expression; gene expression; intracellular receptor-mediated signaling pathway; lymph node development; Peyer's patch development; positive regulation of circadian rhythm; positive regulation of transcription, DNA-dependent; regulation of fat cell differentiation; regulation of steroid metabolic process; steroid hormone mediated signaling; T-helper cell differentiation; transcription initiation from RNA polymerase II promoter; xenobiotic metabolic process

Disease: Immunodeficiency 42