SDS-Page
rnhA recombinant protein
rnhA, 1-155aa, E Coli, His tag, E Coli
Gene Names
rnhA; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
rnhA; 1-155aa; E Coli; His tag; Ribonuclease HI degrades RNA of DNA-RNA hybrids.; Ribonuclease HI; degrades RNA of DNA-RNA hybrids.; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin; rnhA recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT.
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
Sequence Length
155
Applicable Applications for rnhA recombinant protein
SDS-PAGE
Antigen Species
E Coli
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for rnhA recombinant protein
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant E Coli rnhA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for rnhA recombinant protein
References
Nowotny M., et al. (2007) Mol Cell. 28 (2):264-76.; Ten Asbroek AL., et al. (2002) Eur J Biochem. 269 (2):583-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20 kDa (178aa), confirmed by MALDI-TOF
NCBI Official Full Name
ribonuclease HI, degrades RNA of DNA-RNA hybrids
NCBI Official Symbol
rnhA
NCBI Official Synonym Symbols
cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin
NCBI Protein Information
ribonuclease HI, degrades RNA of DNA-RNA hybrids
UniProt Protein Name
Ribonuclease HI
Protein Family
UniProt Gene Name
rnhA
UniProt Synonym Gene Names
dasF; herA; rnh; sdrA; RNase HI; RNase H
NCBI Description
RNase H cleaves RNA in RNA-DNA hybrids. [More information is available at EcoCyc: EG10860].
Uniprot Description
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.
Research Articles on rnhA
Similar Products
Product Notes
The rnhA rnha (Catalog #AAA204532) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's rnhA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the rnhA rnha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV. It is sometimes possible for the material contained within the vial of "rnhA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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