Typical Testing Data/Standard Curve (for reference only)
Rat hepcidin antimicrobial peptide ELISA Kit | HAMP elisa kit
Rat Hepcidin, HAMP ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
may play a role in the regulation of iron metabolism [RGD, Feb 2006]
Uniprot Description
HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; apical cortex; cell; cytoplasm; extracellular region; nucleus
Molecular Function: copper ion binding; hormone activity
Biological Process: iron ion homeostasis; cellular iron ion homeostasis; negative regulation of inflammatory response; myeloid cell homeostasis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; negative regulation of bone resorption; JAK-STAT cascade; defense response to fungus; positive regulation of macrophage activation
Research Articles on HAMP
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Product Notes
The Rat HAMP hamp (Catalog #AAA910836) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA910836 ELISA Kit recognizes Rat HAMP. It is sometimes possible for the material contained within the vial of "hepcidin antimicrobial peptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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