Rabbit anti-Human, Mouse CTSE Polyclonal Antibody | anti-CTSE antibody

CTSE, ID (Cathepsin E, Cathepsin E form I, Cathepsin E form II) (MaxLight 550)

Cat. Num. AAA6467748

• Gene Names: CTSE; CATE
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CTSE; Polyclonal Antibody; ID (Cathepsin E; Cathepsin E form I; Cathepsin E form II) (MaxLight 550); anti-CTSE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CTSE. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 396

• Applicable Applications for anti-CTSE antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CTSE (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTSE antibody (Center) in K562 cell line lysates (35ug/lane).CTSE(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CTSE antibody (Center) in mouse stomach tissue lysates (35ug/lane).CTSE(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CTSE Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CTSE Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CTSE antibody

Antibodies; Cathepsin

References

1. Caruso, M., Virchows Arch. 454(3), 291-302 (2009). 2. Burster, T., Biochem. Biophys. Res. Commun. 377(4), 1299-1303 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4503145
• NCBI GeneID: 1510
• NCBI Accession #: NP_001901
• NCBI GenBank Nucleotide #: NP_001901.1
• UniProt Accession #: P14091
• Molecular Weight: 43.312
• NCBI Official Full Name: cathepsin E isoform a preproprotein
• NCBI Official Synonym Full Names: cathepsin E
• NCBI Official Symbol: CTSE
• NCBI Official Synonym Symbols: CATE
• NCBI Protein Information: cathepsin E
• UniProt Protein Name: Cathepsin E
• Protein Family: Cathepsin
• UniProt Gene Name: CTSE
• UniProt Entry Name: CATE_HUMAN

NCBI Description

This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.34; Protease

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function: protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis