Ribonuclease A7 (RNASE7) Recombinant Protein | RNASE7 recombinant protein

Recombinant Ribonuclease A7 (RNASE7)

Cat. Num. AAA2009152

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ribonuclease A7 (RNASE7); Recombinant Ribonuclease A7 (RNASE7); RNASE7 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-KGMTSSQWFK IQHMQPSPQA CNSAMKNINK HTKRCKDLNT FLHEPFSSVA ATCQTPKIAC KNGDKNCHQS HGAVSLTMCK LTSGKHPNCR YKEKRQNKSY VVACKPPQKK DSQQFHLVPV HLDRV
• Sequence Length: 156

• Applicable Applications for RNASE7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys31~Val155 (Accession # Q9H1E1) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 153252081
• NCBI GeneID: 84659
• NCBI Accession #: NP_115961.2
• NCBI GenBank Nucleotide #: NM_032572.3
• UniProt Accession #: Q9H1E1; P80927; P83685; Q546N3
• Molecular Weight: 15.7kDa
• NCBI Official Full Name: ribonuclease 7
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 7
• NCBI Official Symbol: RNASE7
• NCBI Protein Information: ribonuclease 7; SAP-2; RNase 7; skin-derived antimicrobial protein 2
• UniProt Protein Name: Ribonuclease 7
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASE7
• UniProt Synonym Gene Names: RNase 7; SAP-2
• UniProt Entry Name: RNAS7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein has broad-spectrum antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family.

Protein type: RNA-binding; Secreted; Secreted, signal peptide; Ribonuclease; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region

Molecular Function: peptidoglycan binding; nucleic acid binding; lipopolysaccharide binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; response to bacterium; antibacterial humoral response; innate immune response; membrane disruption in another organism; defense response to Gram-negative bacterium