Human Ribonuclease 7 ELISA Kit | RNASE7 elisa kit

Human Ribonuclease 7 ELISA Kit

Cat. Num. AAA289271

• Gene Names: RNASE7; RAE1
• Reactivity: Human
• Synonyms: Ribonuclease 7; Human Ribonuclease 7 ELISA Kit; RNase 7; Skin-derived antimicrobial protein 2; SAP-2; RNASE7; UNQ2516/PRO6006; RNASE7 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Ribonuclease 7,RNase 7
• Sequence Length: 156

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78 pg/mL - 5000 pg/mL
• Sensitivity: <23 pg/mL
• Intra-assay Precision: <=4.1%
• Inter-assay Precision: <=7.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for RNASE7 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153252081
• NCBI GeneID: 84659
• NCBI Accession #: NP_115961.2
• NCBI GenBank Nucleotide #: NM_032572.3
• UniProt Accession #: Q9H1E1; P80927; P83685; Q546N3
• Molecular Weight: 17,419 Da
• NCBI Official Full Name: ribonuclease 7
• NCBI Official Synonym Full Names: ribonuclease A family member 7
• NCBI Official Symbol: RNASE7
• NCBI Official Synonym Symbols: RAE1
• NCBI Protein Information: ribonuclease 7
• UniProt Protein Name: Ribonuclease 7
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASE7
• UniProt Synonym Gene Names: RNase 7; SAP-2
• UniProt Entry Name: RNAS7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein has broad-spectrum antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; RNA-binding; EC 3.1.27.-; Secreted; Ribonuclease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region

Molecular Function: endonuclease activity; lipopolysaccharide binding; nucleic acid binding; peptidoglycan binding; ribonuclease activity

Biological Process: antibacterial humoral response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; membrane disruption in another organism; response to bacterium