Human Ribonuclease A7 (RNASE7) ELISA Kit | RNASE7 elisa kit

Human Ribonuclease A7 (RNASE7)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709687

• Reactivity: Human
• Applications: ELISA
• Synonyms: Ribonuclease A7 (RNASE7); Human Ribonuclease A7 (RNASE7)(Sandwich ELISA) ELISA Kit; High Sensitive ELISA Kit for Ribonuclease A7 (RNASE7); RNASE7/Ribonuclease A7; SAP-2; Rnase-A7; Ribonuclease; RNase A Family 7; Skin-derived antimicrobial protein 2; RNASE7 elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Ribonuclease A7 (RNASE7); This assay has high sensitivity and excellent specificity for detection of High Sensitive Ribonuclease A7 (RNASE7). No significant cross-reactivity or interference between High Sensitive Ribonuclease A7 (RNASE7) and analogues was observed.

• Applicable Applications for RNASE7 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA-High Sensitive
• Sample Types: tissue homogenates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.59ng/mL
• Assay Length: 3 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Ribonuclease A7 (RNASE7) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Ribonuclease A7 (RNASE7) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for RNASE7 elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 153252081
• NCBI GeneID: 84659
• NCBI Accession #: NP_115961.2
• NCBI GenBank Nucleotide #: NM_032572.3
• UniProt Accession #: Q9H1E1; P80927; P83685; Q546N3
• NCBI Official Full Name: ribonuclease 7
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 7
• NCBI Official Symbol: RNASE7
• NCBI Protein Information: ribonuclease 7; RNase 7; SAP-2; skin-derived antimicrobial protein 2
• UniProt Protein Name: Ribonuclease 7
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASE7
• UniProt Synonym Gene Names: RNase 7; SAP-2
• UniProt Entry Name: RNAS7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein has broad-spectrum antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE7: Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; RNA-binding; EC 3.1.27.-; Secreted; Ribonuclease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region

Molecular Function: endonuclease activity; lipopolysaccharide binding; nucleic acid binding; peptidoglycan binding; ribonuclease activity

Biological Process: antibacterial humoral response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; membrane disruption in another organism; response to bacterium