Human Relaxin 1 ELISA Kit | RLN1 elisa kit

Human Relaxin 1 ELISA Kit

Cat. Num. AAA8248390

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Human
• Synonyms: Relaxin 1; Human Relaxin 1 ELISA Kit; Prorelaxin H1; RLN1 elisa kit

• Reactivity: Human
• Specificity: The Human Relaxin 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Relaxin 1 proteins within the range of 78 pg/ml - 5000 pg/ml.
• Sequence Length: 185

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLN1 elisa kit

Principle of the Assay: The Human Relaxin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Relaxin 1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Relaxin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Relaxin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Relaxin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Relaxin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

NCBI and Uniprot Product Information

• NCBI GI #: 5902052
• NCBI GeneID: 6013
• NCBI Accession #: NP_008842.1
• NCBI GenBank Nucleotide #: NM_006911.3
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: prorelaxin H1 preproprotein
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p24.1