Relaxin 1/RLN1 Recombinant Protein | RLN1 recombinant protein

Relaxin 1/RLN1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120885

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Purity: >82% as determined by SDS-PAGE
• Synonyms: Relaxin 1/RLN1; Relaxin 1/RLN1 Protein; Human; Recombinant (His Tag); Human Relaxin-1/RLN1 Protein (His Tag); bA12D24.3.1 Protein; bA12D24.3.2 Protein; H1 Protein; H1RLX Protein; RLN1 Protein; RLXH1 Protein; relaxin 1; RLN1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >82% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys185

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human RLN1 (NP_008842.1)(Met 1-Cys 185) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for RLN1 recombinant protein

Background: Relaxin-1, also known as Prorelaxin H1 and RLN1, is a secreted protein that belongs to the insulin family. It is a peptide hormone that was first described in 1926 by Frederick Hisaw. Since its discovery as a reproductive hormone 8 years ago, relaxin has been implicated in a number of pregnancy-related functions involving extracellular matrix (ECM) turnover and collagen degradation. It is now becoming evident that relaxin's ability to reduce matrix synthesis and increase ECM degradation has important implications in several nonreproductive organs, including the heart, lung, kidney, liver and skin. The relaxin-like peptide family belongs in the insulin superfamily and consists of 7 peptides of high structural but low sequence similarity; relaxin-1 (RNL1), relaxin-2 (RNL2) and relaxin-3 (RNL3), and the insulin-like (INSL) peptides, INSL3, INSL4, INSL5 and INSL6. The functions of relaxin-3, INSL4, INSL5, INSL6 remain uncharacterised. Relaxin-1/RLN1 is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. Relaxin-1/RLN1 may be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Relaxin and estrogen appear to play protective roles against airway fibrosis, airway SM thickening, and cardiac hypertrophy. Relaxin may also provide a means to regulate excessive collagen deposition during kidney development and in diseased states characterized by renal fibrosis.

References

Bathgate,R.A. et al., 2003, ends Endocrinol Metab 14 (5):207-13. Samuel,C.S. et al., 2003, Curr Opin Pharmacol. 3 (2):152-8. Samuel,C.S. et al., 2004,Kidney Int. 65 (6):2054-64. Lekgabe,E.D. et al., 2006, Endocrinology. 147 (12):5575-83. Samuel,C.S. et al., 2007, Adv Exp Med Biol 612: 88-103.

NCBI and Uniprot Product Information

• NCBI GI #: 5902052
• NCBI GeneID: 6013
• NCBI Accession #: NP_008842.1
• NCBI GenBank Nucleotide #: NM_006911.3
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: prorelaxin H1 preproprotein
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy; signal transduction