Human Relaxin 1 PicoKine ELISA Kit | RLN1 elisa kit

Human Relaxin 1 PicoKine ELISA Kit

Cat. Num. AAA177616

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Human
• Synonyms: Relaxin 1 PicoKine; Human Relaxin 1 PicoKine ELISA Kit; Prorelaxin 1; H1RLX; Preprorelaxin H1; PreprorelaxinH1; Prorelaxin; Prorelaxin H1; ProrelaxinH1; REL1_HUMAN; Relaxin 1 (H1); Relaxin A chain; Relaxin H1; Relaxin1; RelaxinH1; RLN 1; RLN1; RLX H1; RLXH 1; RLXH; relaxin 1; RLN1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Relaxin 1
• Sequence Length: 185

• Assay Range: 78pg/ml-5000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human Relaxin 1 in cell culture supernates, serum and plasma(heparin).
• Immunogen: E Coli, R162-Cys185(A)&K26-S53(B)
• Assay Procedure Step 1: Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each human Relaxin 1 standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|596|1354|2415|688|1542|2229; Standard deviation|29.20|69.05|130.41|35.77|92.52|151.57; CV(%)|4.9|5.1|5.4|5.2|6|6.8
• Sample Data: Concentration(pg/ml)|0|78|156|312|625|1250|2500|5000; O.D.|0.024|0.068|0.101|0.149|0.251|0.540|1.055|1.835
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Relaxin 1 PicoKine ELISA Kit standard curve )

Related Product Information for RLN1 elisa kit

Principle of the assay: Human Relaxin 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 1 has been precoated onto 96-well plates. Standards(E Coli,R162-Cys185 (A) & K26-S53 (B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Relaxin 1 amount of sample captured in plate.

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

NCBI and Uniprot Product Information

• NCBI GI #: 5902052
• NCBI GeneID: 6013
• NCBI Accession #: NP_008842.1
• NCBI GenBank Nucleotide #: NM_006911.3
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: prorelaxin H1 preproprotein
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy; signal transduction