Rat Resistin ELISA Kit | RETN elisa kit

Rat Resistin ELISA Kit

Cat. Num. AAA761946

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Reactivity: Rat
• Synonyms: Resistin; Rat Resistin ELISA Kit; Resistin/RETN/ADSF/Adipose tissue-specific secretory factor/HXCP1/RETN1/RSTNXCP1; RETN elisa kit

• Reactivity: Rat
• Sequence Length: 108

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RETN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Resistin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Resistin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Resistin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Resistincan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 12584202
• NCBI GeneID: 56729
• Molecular Weight: 8,686 Da
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: aging; fat cell differentiation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of synaptic transmission; response to insulin stimulus; response to mechanical stimulus

Disease: Diabetes Mellitus, Noninsulin-dependent