Human Resistin ELISA Kit | RETN elisa kit

Human Resistin PicoKine ELISA Kit

Cat. Num. AAA175924

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Reactivity: Human
• Synonyms: Resistin; Human Resistin PicoKine ELISA Kit; resistin; Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine rich secreted protein A12 alpha like 2; Cysteine rich secreted protein FIZZ3; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ 3; FIZZ3; Found in inflammatory zone 3; HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin delta2; RETN; RETN 1; RETN_HUMAN; RETN1; UNQ407; XCP 1; XCP1; RETN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human RETN
• Sequence Length: 108

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 78 pg/ml - 5,000 pg/ml
• Sensitivity: <3 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RETN elisa kit

Principle of the Assay: The Picokine™ Human RETN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human RETN with a 96-well strip plate that is pre-coated with antibody specific for RETN. The detection antibody is a biotinylated antibody specific for RETN. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human RETN with immunogen: Expression system for standard: E.coli; Immunogen sequence: K19-P108. The kit is analytically validated with ready-to-use reagents. To measure Human RETN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human RETN in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human RETN in the sample.

Background/Introduction: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 18202962
• NCBI GeneID: 56729
• UniProt Accession #: Q9HD89; Q540D9; Q76B53; D6W649
• Molecular Weight: 8,686 Da
• NCBI Official Full Name: Resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging

Disease: Diabetes Mellitus, Noninsulin-dependent