Testing Data
Alpha-fetoprotein ELISA Pair Set | AFP elisa pair set
Alpha-fetoprotein Matched ELISA Antibody Pair Set, Human
Synonyms
Alpha-fetoprotein; Alpha-fetoprotein Matched ELISA Antibody Pair Set; Human; Human AFP/alpha-fetoprotein ELISA Pair Set; alpha-fetoprotein; AFPD Matched ELISA Antibody Pair Set; FETA Matched ELISA Antibody Pair Set; HPAFP Matched ELISA Antibody Pair Set; AFP elisa pair set
Specificity
Quantitative determination of Human Alpha-fetoprotein
Assay Type
Solid Phase Sandwich ELISA
Detection Range
31.25-2000pg/mL
Sensitivity
31.25pg/ml
Materials Provided
1. Capture Antibody 1 mg/mL of mouse anti-Human AFP/alpha-fetoprotein monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human AFP/alpha-fetoprotein monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 115 ng of recombinant Human AFP/alpha-fetoprotein. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human AFP/alpha-fetoprotein monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in Dilution Buffer before use.
3. Standard Each vial contains 115 ng of recombinant Human AFP/alpha-fetoprotein. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for AFP elisa pair set
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
This Alpha-fetoprotein Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Alpha-fetoprotein. It contains Human Alpha-fetoprotein capture antibody, Human Alpha-fetoprotein detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Alpha-fetoprotein protein.
This Alpha-fetoprotein Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Alpha-fetoprotein. It contains Human Alpha-fetoprotein capture antibody, Human Alpha-fetoprotein detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Alpha-fetoprotein protein.
References
Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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Product Notes
The AFP (Catalog #AAA8118424) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Alpha-fetoprotein, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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