Urokinase Plasminogen Activator Receptor Recombinant Active Protein

Urokinase Plasminogen Activator Receptor Recombinant, Human (uPAR, CD87)

Cat. Num. AAA637885

• Applications: ELISA
• Purity: Purified; > 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1 ug of the cytokine as determined by the LAL method.
• Synonyms: Urokinase Plasminogen Activator Receptor Recombinant; Human (uPAR; CD87); Urokinase Plasminogen Activator Receptor Recombinant active protein

• Host: Recombinant protein corresponding to Leu23-Arg303 with a C-Terminal His-Tag from human uPAR, expressed in NSO cells
• Specificity: Recognizes human uPAR.
• Purity/Purification: Purified; > 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1.0 EU per 1 ug of the cytokine as determined by the LAL method.
• Form/Format: Supplied as a lyophilized powder from PBS, containing 50ug of BSA and 1ug carrier protein. Reconstitute with 10ml PBS, 0.1% BSA or HSA to a concentration of 10ug/ml.

• Applicable Applications for Urokinase Plasminogen Activator Receptor Recombinant active protein: ELISA (EL/EIA)
• Application Notes: Optimal dilutions/concentrations should be determined by the end user.
• Endotoxin: <1EU/ug (LAL)
• Biological Activity: Measured by its ability in functional ELISA. Immobilized recombinant uPAR at 5ug/ml (100ul/well) can bind recombinant human uPA with a linear range of 0.3-20ng/ml.
• Preparation and Storage: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Urokinase Plasminogen Activator Receptor Recombinant active protein

Human uPAR. The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is strictly species-specific

Product Categories/Family for Urokinase Plasminogen Activator Receptor Recombinant active protein

Molecular Biology; MB-Cancer Markers

NCBI and Uniprot Product Information

• UniProt Accession #: Q030405