Mouse anti-Chimpanzee CD3 Monoclonal Antibody | anti-CD3 antibody

MOUSE ANTI HUMAN CD3

Cat. Num. AAA224392

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Reactivity: Chimpanzee
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: >95% by SDS-PAGE
• Synonyms: CD3; Monoclonal Antibody; MOUSE ANTI HUMAN CD3; anti-CD3 antibody

• Host: Mouse
• Reactivity: Chimpanzee
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: MEM-57
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 182

• Applicable Applications for anti-CD3 antibody: Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood. ; Flow Cytometry: Minimum Dilution: 1/5; Maximum Dilution: 1/2
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:FITC (MBS210866))

Testing Data #2

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (MBS213740))

Related Product Information for anti-CD3 antibody

Mouse anti Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either delta -ε or gamma -ε subunit complexes (Dave et al. 1997). Mouse anti Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3ε subunit (Transy et al. 1989) as it will recognize murine 3D054.8 hybridoma cells tranfected with cDNA to express the human CDε subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either alpha/beta or gamma /delta expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, gamma, delta, ε and ζ responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006 Dave et al. 1997). Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batista et al. 2004 Brdicková et al. 2003). Clone MEM-57 immunoprecipitates proteins of ~22kDa and more weakly ~28kDa. from peripheral blood T cell lysates.

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064.1
• NCBI GenBank Nucleotide #: NM_000073.2
• Molecular Weight: 20,469 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule, gamma (CD3-TCR complex)
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G
• UniProt Entry Name: CD3G_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3G: The CD3 complex mediates signal transduction.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; T cell receptor binding

Biological Process: regulation of immune response; protein transport; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17