Western Blot (WB)
(Western Blot: Sample: Recombinant CD27BP, Human.)
Rabbit anti-Human CD27 Binding Protein Polyclonal Antibody | anti-CD27BP antibody
Polyclonal Antibody to CD27 Binding Protein (CD27BP)
Gene Names
SIVA1; SIVA; CD27BP; Siva-1; Siva-2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
CD27 Binding Protein; Polyclonal Antibody; Polyclonal Antibody to CD27 Binding Protein (CD27BP); anti-CD27BP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CD27BP. It has beenselected for its ability to recognize CD27BP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPKRSCPFAD VAPLQLKVRV SQRELSRGVC AERYSQEVFE KTKRLLFLGA QAYLDHVWDE GCAVVHLPES PKPGPTGAPR AARGQMLIGP DGRLIRSLGQ ASEADPSGVA SIACSSCVRA VDGKAVCGQC ERALCGQCVR
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPKRSCPFAD VAPLQLKVRV SQRELSRGVC AERYSQEVFE KTKRLLFLGA QAYLDHVWDE GCAVVHLPES PKPGPTGAPR AARGQMLIGP DGRLIRSLGQ ASEADPSGVA SIACSSCVRA VDGKAVCGQC ERALCGQCVR
Applicable Applications for anti-CD27BP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
CD27BP (Met1~Thr175)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant CD27BP (Met1~Thr175) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083773
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant CD27BP, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CD27BP, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,778 Da
NCBI Official Full Name
apoptosis regulatory protein Siva isoform 1
NCBI Official Synonym Full Names
SIVA1 apoptosis inducing factor
NCBI Official Symbol
SIVA1
NCBI Official Synonym Symbols
SIVA; CD27BP; Siva-1; Siva-2
NCBI Protein Information
apoptosis regulatory protein Siva
UniProt Protein Name
Apoptosis regulatory protein Siva
UniProt Gene Name
SIVA1
UniProt Synonym Gene Names
SIVA; CD27BP
NCBI Description
This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Siva: a proapoptotic protein. Associates with CD27, a member of the tumor necrosis factor receptor family. Two alternatively spliced isoforms have been described. Isoform 1 but not isoform 2 may induce TNFRSF7/CD27 mediated apoptosis. Isoform 1 inhibits Bcl-xL anti-apoptotic activity.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: cytoplasm; nucleoplasm
Molecular Function: CD27 receptor binding; metal ion binding; protein binding; tumor necrosis factor receptor binding; viral receptor activity
Biological Process: entry of virus into host cell
Research Articles on CD27BP
Similar Products
Product Notes
The CD27BP siva1 (Catalog #AAA2028128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to CD27 Binding Protein (CD27BP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 Binding Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CD27BP siva1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPKRSCPFAD VAPLQLKVRV SQRELSRGVC AERYSQEVFE KTKRLLFLGA QAYLDHVWDE GCAVVHLPES PKPGPTGAPR AARGQMLIGP DGRLIRSLGQ ASEADPSGVA SIACSSCVRA VDGKAVCGQC ERALCGQCVR. It is sometimes possible for the material contained within the vial of "CD27 Binding Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
