RBP4 recombinant protein

Recombinant Mouse RBP4 Protein (His tag)

Cat. Num. AAA2547126

• Gene Names: Rbp4; Rbp-4
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: RBP4; Recombinant Mouse RBP4 Protein (His tag); Rbp-4; Rbp4; RBP4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 201

• Application Notes: The secreted recombinant mouse RBP4 comprises 194 amino acids and has a predicted molecular mass of 22.8 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 19
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for RBP4 recombinant protein

Background: Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

Description: A DNA sequence encoding the mouse RBP4 (NP_035385.1) (Met 1-Leu 201) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 33859612
• NCBI GeneID: 19662
• NCBI Accession #: NP_035385.1
• NCBI GenBank Nucleotide #: NM_011255.3
• UniProt Accession #: Q00724; P70357; Q566I5
• Molecular Weight: 23,206 Da
• NCBI Official Full Name: retinol-binding protein 4 isoform 2
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: Rbp-4
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: Rbp4
• UniProt Synonym Gene Names: PRBP; RBP

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19 C2|19 32.75 cM

Cellular Component: extracellular space; protein complex

Molecular Function: protein binding; protein heterodimerization activity; retinol binding

Biological Process: cardiac muscle development; detection of light stimulus involved in visual perception; embryonic development; embryonic organ morphogenesis; embryonic retina morphogenesis in camera-type eye; embryonic skeletal development; eye development; female genitalia morphogenesis; gluconeogenesis; glucose homeostasis; heart development; lung development; maintenance of gastrointestinal epithelium; male gonad development; negative regulation of cardiac muscle cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of insulin secretion; response to insulin stimulus; response to retinoic acid; retina development in camera-type eye; retinal metabolic process; retinol metabolic process; spermatogenesis; urinary bladder development; uterus development; vagina development