Western Blot (WB)
(PHF20 Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the PHF20 antibody detected the PHF20 protein (arrow).)
Rabbit PHF20 Polyclonal Antibody | anti-PHF20 antibody
PHF20 Antibody (N-term)
Western Blot (WB)
(PHF20 Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the PHF20 antibody detected the PHF20 protein (arrow).)
Western Blot (WB)
(PHF20 Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the PHF20 antibody detected the PHF20 protein (arrow).)
Immunohistochemistry (IHC)
(PHF20 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHF20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PHF20 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PHF20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Heisel, S.M., et al. PLoS ONE 3 (5), E2164 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Pallasch, C.P., et al. Int. J. Cancer 117(3):456-459(2005)
Dou, Y., et al. Cell 121(6):873-885(2005)
NCBI and Uniprot Product Information
Uniprot Description
PHF20: Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA-binding; Nuclear envelope; Acetyltransferase
Chromosomal Location of Human Ortholog: 20q11.22-q11.23
Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; histone acetyltransferase complex
Molecular Function: protein binding; DNA binding; zinc ion binding; histone acetyltransferase activity (H4-K16 specific)
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on PHF20
Similar Products
Product Notes
The PHF20 phf20 (Catalog #AAA9201491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 123-151. The PHF20 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's PHF20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PHF20 phf20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
