Rabbit anti-Mouse RBP4 Monoclonal Antibody | anti-RBP4 antibody

Recombinant Anti-RBP4 Antibody, Rabbit Monoclonal

Cat. Num. AAA8111133

• Gene Names: Rbp4; Rbp-4
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: RBP4; Monoclonal Antibody; Recombinant Anti-RBP4 Antibody; Rabbit Monoclonal; RBP4 Antibody; Rabbit MAb; retinol binding protein 4; plasma; Anti-Rbp-4 Antibody; anti-RBP4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Mouse RBP4.; Has cross-reactivity in ELISA with Human RBP4; Has cross-reactivity in ELISA with Human RBP4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RBP4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse RBP4 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse RBP4 (rM RBP4).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RBP4 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:23 kDa)

Related Product Information for anti-RBP4 antibody

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

References

Yang Q, et al. (2005) Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 436(7049): 356-62.Choi SH, et al. (2008) High plasma retinol binding protein-4 and low plasma adiponectin concentrations are associated with severity of glucose intolerance in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 93(8): 3142-8.Tepper BJ, et al. (2010) Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes. Clin Biochem. 43(3): 320-3.

NCBI and Uniprot Product Information

• NCBI GI #: 33859612
• NCBI GeneID: 19662
• NCBI Accession #: NP_035385.1
• NCBI GenBank Nucleotide #: NM_011255.3
• UniProt Accession #: Q00724; P70357; Q566I5
• Molecular Weight: 23,206 Da
• NCBI Official Full Name: retinol-binding protein 4 isoform 2
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: Rbp-4
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol binding protein 4, cellular
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: Rbp4
• UniProt Synonym Gene Names: PRBP; RBP

Uniprot Description

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.