Rabbit S-phase Kinase-associated Protein 2 Polyclonal Antibody

S-phase Kinase-associated Protein 2 (SKP2, Cyclin-A/CDK2-associated Protein p45, F-box Protein Skp2, F-box/LRR-repeat Protein 1, FBL1, FBXL1, FLB1, MGC1366, p45skp2)

Cat. Num. AAA620954

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: S-phase Kinase-associated Protein 2; Polyclonal Antibody; S-phase Kinase-associated Protein 2 (SKP2; Cyclin-A/CDK2-associated Protein p45; F-box Protein Skp2; F-box/LRR-repeat Protein 1; FBL1; FBXL1; FLB1; MGC1366; p45skp2); Anti -S-phase Kinase-associated Protein 2 (SKP2; anti-S-phase Kinase-associated Protein 2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SKP2. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.

• Applicable Applications for anti-S-phase Kinase-associated Protein 2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry (Paraffin and Formalin): 1.2ug/ml
• Immunogen: Synthetic peptide corresponding to aa2-20, HRKHLQEIPDLSSNVATSF to the N-terminus of human S-phase kinase-associated protein 2.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with 100ul sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded human mammary cancer sections using rabbit polyclonal antibody to human S-phase kinase-associated protein 2 (1-2 μg/ml). Secondary biotinylated goat anti-rabbit IgG antibody (10 μg/ml).)

Related Product Information for anti-S-phase Kinase-associated Protein 2 antibody

S-phase kinase-associated protein 2 (SKP2) is a subunit in the SKP1-CUL1-F-box protein E3 ubiquitin protein ligase complex. This complex mediates the ubiquitination and the subsequent degradation of proteins involved in cell cycle progression, signal transduction and transcription.

Product Categories/Family for anti-S-phase Kinase-associated Protein 2 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 68161558
• NCBI Official Full Name: S-phase kinase associated protein 2
• Protein Family: S-phase kinase-associated protein

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]