Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) Standard Curve.)
Rat Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4) ELISA Kit | TNFRSF4 elisa kit
Rat Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4) ELISA Kit
Gene Names
Tnfrsf4; Ox40; Txgp1
Reactivity
Rat
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4); Rat Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4) ELISA Kit; TNFRSF4; RP5-902P8.3; ACT35; CD134; OX40; TXGP1L; ATC35 antigen; CD134 antigen; OX40 antigen; OX40 cell surface antigen; OX40 homologue; lymphoid activation antigene ACT35; tax-transcriptionally activated glycopro; TNFRSF4 elisa kit
Reactivity
Rat
Specificity
Rat Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFRSF4. No significant cross-reactivity or interference between Rat TNFRSF4 and analogues was observed.
Sequence Length
271
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) Standard Curve.)
Related Product Information for TNFRSF4 elisa kit
Description: This Rat Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF4 in samples. An antibody specific for TNFRSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFRSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This protein is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
Background: This protein is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 4
NCBI Official Synonym Full Names
TNF receptor superfamily member 4
NCBI Official Symbol
Tnfrsf4
NCBI Official Synonym Symbols
Ox40; Txgp1
NCBI Protein Information
tumor necrosis factor receptor superfamily member 4
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 4
Protein Family
UniProt Gene Name
Tnfrsf4
UniProt Synonym Gene Names
Ox40; Txgp1l
UniProt Entry Name
TNR4_RAT
NCBI Description
membrane-bound receptor, binds adaptor proteins Traf2 and Traf5; member of the tumor necrosis factor (TNF)-receptor superfamily [RGD, Feb 2006]
Uniprot Description
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity ().
Research Articles on TNFRSF4
Similar Products
Product Notes
The Rat TNFRSF4 tnfrsf4 (Catalog #AAA9711673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711673 ELISA Kit recognizes Rat TNFRSF4. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily Member 4 (TNFRSF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
