Rabbit anti-Human NFAT2/NFATC1 Polyclonal Antibody | anti-NFATC1 antibody

Anti-NFAT2/NFATC1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101051

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NFAT2/NFATC1; Polyclonal Antibody; Anti-NFAT2/NFATC1 Antibody; Rabbit Polyclonal; NFATC1 Antibody; Rabbit PAb; Antigen Affinity Purified; nuclear factor of activated T-cells; cytoplasmic; calcineurin-dependent 1; Anti-NF-ATC Antibody; Anti-NFAT2 Antibody; Anti-NFATc Antibody; anti-NFATC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NFATC1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NFATC1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human NFATC1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human NFATC1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NFATC1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: THP1 Whole Cell LysateLane D: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:101 kDaObserved band size:101 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of NFATC1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NFATC1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(NFAT2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-NFAT2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NFAT2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 101 kDa)

Related Product Information for anti-NFATC1 antibody

The nuclear factor of the activated T cell (NFAT) family of transcription factors regulates cytokine gene expression by binding to the promoter/enhancer regions of antigen-responsive genes, usually in cooperation with heterologous DNA-binding partners. The nuclear factor of activated T lymphocytes (NFATC1) signaling has been demonstrated to play important roles in cardiac valve and septal development. Genetic variants in genes involved in NFATC1 signaling may affect their expression and promote the formation of congenital heart disease (CHD). Nuclear factor of activated T cells (NFATC1) was up-regulated in cancers mediating malignant behaviors. The nuclear factor of activated T cell 1 (Nfatc1) locus is a common insertion site for murine tumorigenic retroviruses, suggesting a role of transcription factor NFATc1 in lymphomagenesis.

Product Categories/Family for anti-NFATC1 antibody

Non-Canonical Wnt Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 8928220
• NCBI GeneID: 4772
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• NCBI Official Full Name: Nuclear factor of activated T-cells, cytoplasmic 1
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc
• UniProt Entry Name: NFAC1_HUMAN

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; mitogen-activated protein kinase p38 binding; FK506 binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; calcium ion transport; heart development; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; G1/S transition of mitotic cell cycle