Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit | Pik3ca elisa kit

Mouse Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit

Cat. Num. AAA2882390

• Gene Names: Pik3ca; p110; caPI3K; p110alpha; 6330412C24Rik
• Reactivity: Mouse
• Synonyms: Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit alpha isoform; Mouse Phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Pik3ca elisa kit

• Reactivity: Mouse
• Sequence Length: 1068

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.39 ng/mL
• Intra-Assay CV: <=6.2%
• Inter-Assay CV: <=11.3%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 254540080
• NCBI GeneID: 18706
• NCBI Accession #: NP_032865.2
• NCBI GenBank Nucleotide #: NM_008839.2
• UniProt Accession #: P42337; Q0VGQ5
• Molecular Weight: 124,412 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• NCBI Official Symbol: Pik3ca
• NCBI Official Synonym Symbols: p110; caPI3K; p110alpha; 6330412C24Rik
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: Pik3ca
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
• UniProt Entry Name: PK3CA_MOUSE

Uniprot Description

PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.

Protein type: Oncoprotein; Carbohydrate Metabolism - inositol phosphate; EC 2.7.11.1; Kinase, lipid; EC 2.7.1.153; Motility/polarity/chemotaxis

Cellular Component: lamellipodium; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; kinase activity; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; protein kinase activator activity; protein serine/threonine kinase activity; transferase activity

Biological Process: adaptive immune response; angiogenesis; glucose metabolic process; inflammatory response; innate immune response; liver development; negative regulation of neuron apoptosis; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; phosphorylation; platelet activation; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cellular respiration; regulation of gene expression; regulation of multicellular organism growth